Manuscript: Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells

This 2018 Publication describes early work leading to establishment of RepliGut® Systems.  In this manuscript, Dr. Yuli Wang and colleagues detail isolation of human stem cells and establishment of self-renewing monolayers of intestinal epithelial stem cells.

https://www.cmghjournal.org/article/S2352-345X(17)30046-2/fulltext

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Why GI Toxicity Slips Through Preclinical Screening

Why GI Toxicity Slips Through Preclinical Screening

Ask a program lead which toxicity they are most afraid of finding late, and the gut is rarely the first…
Well-to-Well Consistency: Seeding, Confluence, and Real Signal vs Noise

Well-to-Well Consistency: Seeding, Confluence, and Real Signal vs Noise

Some of the most damaging variability in a gut assay never involves the drug at all. It lives between neighboring…
Reproducibility in Your Hands: Why an Assay Drifts When It Moves Between Labs

Reproducibility in Your Hands: Why an Assay Drifts When It Moves Between Labs

A model can be perfectly reproducible in the lab that built it and still fall apart the moment it lands…