Human Custom Gut Assay Services.
Our custom gut assay services put PhD level project leads and RepliGut® human tissue behind your compound. So you get reproducible, decision ready data.
Studies designed around your compound, run by the scientists advising you
Whether you know exactly what you want or need our guidance, our PhD level project leads help you get the most from RepliGut® models. They design the study, choose the region of interest, explore our donor biobank, and select the endpoints that answer your question.
Because the people advising you also run the assays, you get realistic timelines and frequent updates. In practice, that means efficient project design, full visibility into ongoing work, and high success rates built on real experience.
Human cell diversity in a single monolayer
Because RepliGut® grows from primary intestinal stem cells, the differentiated layer contains the epithelial lineages found in vivo. That includes absorptive enterocytes, mucus producing goblet cells, and hormone secreting enteroendocrine cells.
Grown submerged on a semi permeable membrane, RepliGut® Planar cultures open both the apical and basal surfaces. So you can dose one side and sample the other, exactly as absorption and secretion work in the body.
Explore RepliGut® Planar
The engineered insert, with a magnified cross-section of one crypt unit forming over a single microhole.
Reach the stem cell niche with RepliGut® Crypt
Some questions live at the base of the crypt, where stem cells drive renewal and repair. RepliGut® Crypt answers them with an engineered insert, unlike a standard Transwell. Its scaffold is laser drilled with a hexagonal array of microholes. Growth factors rise through each microhole, so stem cells stay proliferative above it while neighboring cells differentiate toward the edges. That recreates the true crypt architecture, stem cells at the base and mature cells around them.
So you can study secretory, proliferative, and absorptive lineages in one culture. You can also track wound healing kinetics as new cells repopulate a gap. Many programs pair Crypt with RepliGut® Planar, one for mechanism, one for throughput.
Explore RepliGut® CryptWhat custom gut assay services let you study
RepliGut® cultures grow polarized human intestinal epithelium on semi permeable membranes. So you can run many study types on one reproducible platform, each designed with your project lead.
Measure barrier function
Track epithelial integrity with high TEER, then screen compounds for their potential to disrupt the barrier.
Model inflammatory response
Dose and sample cytokines apically or basally to study gut inflammation. See InflammaScreen
Screen for GI toxicity
Flag drug induced GI toxicity early, before Phase 1 trials. See StemTox
Study drug disposition
Assess absorption, permeability, and metabolism across intestinal regions. See In Vitro DMPK
Standardized readouts for reliable data
These are the readouts your program can draw on. Available endpoints depend on the model, so your project lead matches them to your study. Each one is standardized for reproducibility across donors and runs.
Human relevant data also fits where regulators are heading. The FDA now actively advances New Approach Methodologies for preclinical testing.
Ready to design your study?
Tell us what you are trying to answer. Our project leads will scope the right custom gut assay services for your program, then run them on real human tissue.
Start your free consultation