The RepliGut® model is a unique, human stem cell-derived platform that recreates the intestinal epithelium in a continuous layer of stem and differentiated cells. RepliGut® system can be purchased as a kit, or our team of experts can provide the services you require.
The high throughput platform supports quick screening of candidates and fosters efficient development through reduced timelines and costs by incorporating human-relevant intestinal epithelial cells. Focus applications include preclinical studies for compound screening, investigating the effects of diseases, and microbiome research.
The RepliGut® Difference
Replicates human biology
using ethically sourced donor-derived intestinal epithelial cells to support development of safer, more effective drugs.
Reduces the need for animal-based preclinical studies for compound screening, disease modeling, and microbiome research.
Reduces downstream timelines and costs involved in clinical trials by screening for more effective drug candidates
More accurate and reproducible data as a basis for better success rates in clinical trials and beyond.
Innovators, outsource research to Altis or buy RepliGut® kits
The monolayer format produces a continuous layer of stem or differentiated cells on a flat and porous surface.
Each tissue sample on the patent-pending RepliGut® kit features a biomimetic scaffold, which separates RepliGut® cells from the cassette’s porous membrane and allows RepliGut® cells to survive for a prolonged period. Without the RepliGut® scaffold, stem cells cannot survive on the cassette.
Luminal and basal reservoirs allow compounds and additional cell types to interact with the epithelial cells for side specific assays.
RepliGut® Planar was developed to replace Caco-2 cells (cancer cells) and animal testing, which are common components of preclinical compound screening. This 2D monolayer platform not only provides better results than immortalized cell lines, as it uses proliferative stem cells and terminally differentiated intestinal epithelial cells, but also provides more physiologically relevant results than using animals
RepliGut® kits are available in both 12-well and 96-well transwell plate formats to accommodate scientist's needs. Each transwell is pre-coated with extracellular matrix designed for culturing these specialized intestinal epithelial cells.
RepliGut® kits are sold complete with the cells, plates, and media needed to culture this unique human intestinal epithelial model in your own facility.
RepliGut® tissue constructs are polarized monolayers that express tight junction proteins and can be tailored to be comprised of stem/progenitor cells, differentiated cells, or both. All major cell lineages in physiologic ratios can be achieved.
Unrivaled biobank with multiple donors, allows customers to choose regional specificity of the small intestine and colon from donors of different demographic backgrounds to further enhance study data. Fit-for-purpose specimens, aligned with scientific objectives, and the guaranteed ability to work with the same donor for years.
Different demographic backgrounds to further enhance study data
Guaranteed ability to work with the same donor for years
Multiple regions available from small intestine and colon
Access to primary cells and the crypts
Fit-for-purpose specimens, aligned with scientific objectives
Ethics Committee approved organ procurement
More platforms currently under development.
To purchase a RepliGut® Kit, please contact us.