Literature and Articles

Browse peer-reviewed literature, posters, webinars, blog articles, and more showing how we and others are using RepliGut Systems to support discovery.

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Manuscript: NSAID-Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells
Poster: Characterization of biological variability in a stem cell-derived human intestinal epithelium model
Poster: Genomic evaluation of RepliGut Planar Jejunum in Response to metabolic Inducers
Poster Presented at ISSX/DMDG meeting – June 2023
Poster: Genomic evaluation of RepliGut Planar Jejunum in response to metabolic inducers
Inflammation

Scientist Jarrett Bilton, PhD recently travelled to the 25th North American ISSX conference in Boston. In collaboration with researchers at Genentech, RepliGut® Planar Jejunum was evaluated for gene expression patterns in response to prototypical metabolic inducers Rifampin, CITCO, 3-MC, and Calcitriol.

Poster: Characterization of biological variability in a stem cell-derived human intestinal epithelium model
Inflammation

Associate Director Liz Boazak recently traveled to the 2nd MPS World Summit held in Berlin, Germany June 25-29, 2023.  Her poster was presented in the Reproducibility topic session and describes characterizing multiple donor and batch statistics of baseline RepliGut® Planar- transverse colon. 

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Poster: Characterization of biological variability in a stem cell-derived human intestinal epithelium model
Inflammation
Associate Director Liz Boazak recently travelled to the 2nd MPS World Summit held in Berlin, Germany June 25-29, 2023. Her poster was presented in the Reproducibility topic session and describes characterizing multiple donor and batch statistics of baseline RepliGut® Planar- transverse colon. Poster further describes how minimizing the variability improves ability to discern true biological responses to inflammatory insults.
Manuscript: Evaluation of human primary intestinal monolayers for drug metabolizing capabilities
ADME
In this 2019 manuscript, Dr. Jennifer Speer and colleagues describe levels of CYP3A4, UGTs 2B17, 1A1 and 1A10, and CES2 proteins in freshly isolated intestinal crypts compared to 2 different monolayer intestinal epithelium formats derived from intestinal crypt stem cells. this work highlights the importants of the mechanical properties of the cell scaffold in maintaining intestinal epithelial cell metabolic functionality.
Manuscript: Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells
GI Toxicity, Inflammation, ADME
This 2018 Publication describes early work leading to establishment of RepliGut® Systems. In this manuscript, Dr. Yuli Wang and colleagues detail isolation of human stem cells and establishment of self-renewing monolayers of intestinal epithelial stem cells.
Poster: GB004 Exhibits Protective Effects Directly on Epithelial Cells Using Ex Vivo Organoid and Monolayer Cultures
Inflammation
Client Kristin Taylor Meadows and colleagues from Gossamer Bio used RepliGut® Planar to established conditions for inducing inflammation response using TNFα. Using this model, they show how GB-004, a novel compound in development for treating ulcerative colitis reduced the destructive effects of TNFα on epithelial barrier integrity.
Manuscript: Analysis of Interleukin 8 Secretion by a Stem-Cell-Derived Human Intestinal Epithelial Monolayer Platform
Inflammation
Authors Yuli Wang and colleagues describe measurement of 8 cytokines released from stem cell-derived intestinal epithelium in response to exposure to the pro-inflammatory cytokine TNFa. The article shows results from using stem cells from 5 different regions of the intestine. The most responsive cytokine was IL-8 in all regions which could be blocked using common anti-inflammatory factors present in the gut.
Manuscript: NSAID-Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells.
GI Toxicity
Author Andrea Bhatt and colleagues used monolayer of intestinal epithelial cells to show that exposure to NSAIDs at physiologically relevant doses can cause changes in permeability in small intestine. This article highlights the potential utility of RepliGut® Planar for assessing toxicity of dietary factors, bacterial metabolites, or therapeutics.
Altis enters strategic partnership with CN-Bio
The Next Big Thing in Pharma
Poster: Characterization of a novel human primary intestinal epithelial monolayer platform in response to a cytokine challenge
How Does Altis Envision the Future of the Pharma Industry?
Manuscript: Analysis of Interleukin 8 Secretion by a Stem-Cell-Derived Human Intestinal Epithelial Monolayer Platform
Poster: GB004 Exhibits Protective Effects Directly on Epithelial Cells Using Ex Vivo Organoid and Monolayer Cultures
Manuscript: Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells
Manuscript: Evaluation of human primary intestinal monolayers for drug metabolizing capabilities
Poster: Primary Human Small Intestine Monolayers For Assessing Drug-Induced Intestinal Liabilities In Vitro
Poster: A Sensitive and Specific Human Primary Stem Cell-Based In Vitro Assay for Predicting The Gastrointestinal Toxicity Risk of Therapeutic Agents
Poster: A primary jejunum and primary hepatocyte multi-organ MPS: a promising tool for more predictive studies of human drug ADME and oral bioavailability
Altis Biosystems Appoints Jimmy Smedley, Ph.D. Chief Operating Officer
Manuscript: NSAID-Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells
Poster: Characterization of biological variability in a stem cell-derived human intestinal epithelium model
Poster: Genomic evaluation of RepliGut Planar Jejunum in Response to metabolic Inducers
How Does Altis Biosystems Envision the Future of the Pharma/Biopharma Industry?
Assessing Drug-induced Gut Toxicity In Vitro
Predicting Clinical GI Safety Outcomes Using an in vitro Human Intestinal Epithelial Model
Poster Presented at ISSX/DMDG meeting – June 2023
A Novel, High-Throughput Platform to Characterize Novel IBD Therapeutics

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