Clara Jundt and her team at Janssen R&D, in collaboration with Altis and UNC-Chapel Hill, conducted a study using human primary intestinal epithelial cells from multiple donors. Their aim was to determine if these cells could mimic a similar response and capture the genetic variability found in humans. The researchers treated the models with TNFα […]
Authors Yuli Wang and colleagues describe interleukin-8 secretion analysis. This includes the measurement of 8 cytokines released from stem cell-derived intestinal epithelium. The analysis occurs in response to exposure to the pro-inflammatory cytokine TNFa. The article shows results from using stem cells from 5 different regions of the intestine. The most responsive cytokine was IL-8
This 2018 Publication describes early work leading to establishment of RepliGut® Systems. In this manuscript, Dr. Yuli Wang and colleagues detail isolation of human stem cells and establishment of self-renewing monolayers of intestinal epithelial stem cells. https://www.cmghjournal.org/article/S2352-345X(17)30046-2/fulltext
Manuscript: Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells Read More »
In this 2019 manuscript, Dr. Jennifer Speer and colleagues examine human primary intestinal monolayers. They describe levels of CYP3A4, UGTs 2B17, 1A1 and 1A10, and CES2 proteins in freshly isolated intestinal crypts. They compare these levels to 2 different monolayer intestinal epithelium formats derived from intestinal crypt stem cells. This work highlights the importance of
The RepliGut SOT 2023 poster, presented at the 2023 SOT meeting, features collaborators from Janssen Pharmaceuticals describing their use of the RepliGut® Planar Platform to distinguish between compounds with different potencies for the JNJ epigenetic target X with a known in vivoGI liability. The poster describes testing a limited set of reference compounds including drugs with a
GI Toxicities are the most common drug-induced adverse events in human clinical trials. Unfortunately, they are poorly predicted by rodent models. View our poster presentation from the 2023 SOT meeting authored by James Levi. It describes how a new human-derived cell model provides an efficient and informative screen for drug effects on intestinal cell proliferation. In
In a partnership with CN-Bio, Dr. Yassen Abbas, Senior Scientist, highlighted the effectiveness of fluidically connected liver tissue. He showed how the RepliGut® Planar-Jejunum determines the impact of intestinal epithelium on drug bioavailability and disposition. The team presented their research in a poster in June of 2023 at the EU ISSX/DMDG meeting. A primary jejunum