Authors Yuli Wang and colleagues describe interleukin-8 secretion analysis. This includes the measurement of 8 cytokines released from stem cell-derived intestinal epithelium. The analysis occurs in response to exposure to the pro-inflammatory cytokine TNFa. The article shows results from using stem cells from 5 different regions of the intestine. The most responsive cytokine was IL-8 in all regions. Notably, this response could be blocked using common anti-inflammatory factors present in the gut.
Manuscript: Analysis of Interleukin 8 Secretion by a Stem-Cell-Derived Human Intestinal Epithelial Monolayer Platform
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