Authors Yuli Wang and colleagues describe interleukin-8 secretion analysis. This includes the measurement of 8 cytokines released from stem cell-derived intestinal epithelium. The analysis occurs in response to exposure to the pro-inflammatory cytokine TNFa. The article shows results from using stem cells from 5 different regions of the intestine. The most responsive cytokine was IL-8 in all regions. Notably, this response could be blocked using common anti-inflammatory factors present in the gut.
Manuscript: Analysis of Interleukin 8 Secretion by a Stem-Cell-Derived Human Intestinal Epithelial Monolayer Platform
Ready to design your study?
Well-to-Well Consistency: Seeding, Confluence, and Real Signal vs Noise
Some of the most damaging variability in a gut assay never involves the drug at all. It lives between neighboring…
Reproducibility in Your Hands: Why an Assay Drifts When It Moves Between Labs
A model can be perfectly reproducible in the lab that built it and still fall apart the moment it lands…
Biological vs Technical Replicates: Powering a Gut Assay You Can Trust
Here is a mistake that survives peer review more often than it should. A team runs an experiment once, measures…