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2022
Bolster, Doug; Chae, Lee; van Klinken, Jan-Willem; Kalgaonkar, Swati
Impact of selected novel plant bioactives on improvement of impaired gut barrier function using human primary cell intestinal epithelium: Journal Article
In: Journal of Food Bioactives, vol. 20, 2022, ISSN: 2637-8779.
Abstract | Links | BibTeX | Tags: Bioactives, Gut barrier function, Gut permeability, Hepatic nuclear factor 4α, In vitro model, inflammatory bowel disease, intestinal barrier, N-Trans-caffeoyltyramine, N-Trans-feruloyltyramine
@article{bolster_impact_2022,
title = {Impact of selected novel plant bioactives on improvement of impaired gut barrier function using human primary cell intestinal epithelium:},
author = {Doug Bolster and Lee Chae and Jan-Willem van Klinken and Swati Kalgaonkar},
url = {http://www.isnff-jfb.com/index.php/JFB/article/view/301},
doi = {10.31665/JFB.2022.18324},
issn = {2637-8779},
year = {2022},
date = {2022-12-30},
urldate = {2022-12-30},
journal = {Journal of Food Bioactives},
volume = {20},
abstract = {Gut barrier function is compromised in the obese state. The N-trans caffeoyltyramine (NCT) and N-trans feruloyltyramine (NFT), two naturally occurring bioactive compounds in hemp hulls, identified using in silico approaches, have the potential to improve gut barrier function and their effects were studied here in vitro. Proliferative human transverse colon epithelial cells were plated and co-cultured with tumor necrosis factor (TNF) along with NCT, NFT or NCT/NFT (2.2 ratio) post-differentiation, over a 48-hour period to induce inflammation and to observe the effects of NCT and NFT. A decrease in transepithelial electrical resistance (TEER) and increase in the intestinal permeability were observed with increased addition of TNF. Co-administration of NCT and NFT demonstrated a dose-dependent and statistically significant reversal of impaired TEER and intestinal permeability. NCT and NFT demonstrated a physiologically relevant reversal of impaired gut barrier function in the setting of inflammation via significant improvement in TEER and percent permeability.},
keywords = {Bioactives, Gut barrier function, Gut permeability, Hepatic nuclear factor 4α, In vitro model, inflammatory bowel disease, intestinal barrier, N-Trans-caffeoyltyramine, N-Trans-feruloyltyramine},
pubstate = {published},
tppubtype = {article}
}
Gut barrier function is compromised in the obese state. The N-trans caffeoyltyramine (NCT) and N-trans feruloyltyramine (NFT), two naturally occurring bioactive compounds in hemp hulls, identified using in silico approaches, have the potential to improve gut barrier function and their effects were studied here in vitro. Proliferative human transverse colon epithelial cells were plated and co-cultured with tumor necrosis factor (TNF) along with NCT, NFT or NCT/NFT (2.2 ratio) post-differentiation, over a 48-hour period to induce inflammation and to observe the effects of NCT and NFT. A decrease in transepithelial electrical resistance (TEER) and increase in the intestinal permeability were observed with increased addition of TNF. Co-administration of NCT and NFT demonstrated a dose-dependent and statistically significant reversal of impaired TEER and intestinal permeability. NCT and NFT demonstrated a physiologically relevant reversal of impaired gut barrier function in the setting of inflammation via significant improvement in TEER and percent permeability.