Publications

@article{thomas_clostridioides_2025,

title = {Clostridioides difficile toxins alter host metabolic pathway and bile acid homeostasis gene expression in colonic epithelium},

author = {Stephanie A. Thomas and Colleen M. Pike and Cypress E. Perkins and Sean T. Brown and Xochilt M. Espinoza Jaen and Arthur S. McMillan and Casey M. Theriot},

url = {https://journals.asm.org/doi/full/10.1128/iai.00150-25},

doi = {10.1128/iai.00150-25},

year  = {2025},

date = {2025-06-30},

urldate = {2025-06-30},

journal = {Infection and Immunity},

volume = {93},

number = {8},

pages = {e00150–25},

note = {Publisher: American Society for Microbiology},

keywords = {intestinal microenvironment, microbiome},

pubstate = {published},

tppubtype = {article}

}

@article{magnusson_exposure_2024,

title = {Exposure of Colon-Derived Epithelial Monolayers to Fecal Luminal Factors from Patients with Colon Cancer and Ulcerative Colitis Results in Distinct Gene Expression Patterns},

author = {Maria K. Magnusson and Anna Bas Forsberg and Alexandra Verveda and Maria Sapnara and Julie Lorent and Otto Savolainen and Yvonne Wettergren and Hans Strid and Magnus Simrén and Lena Öhman},

url = {https://www.mdpi.com/1422-0067/25/18/9886},

doi = {10.3390/ijms25189886},

issn = {1422-0067},

year  = {2024},

date = {2024-09-13},

urldate = {2024-09-13},

journal = {Int. J. Mol. Sci.},

volume = {25},

number = {18},

pages = {9886},

abstract = {Microbiota and luminal components may affect epithelial integrity and thus participate in the pathophysiology of colon cancer (CC) and inflammatory bowel disease (IBD). Therefore, we aimed to determine the effects of fecal luminal factors derived from patients with CC and ulcerative colitis (UC) on the colonic epithelium using a standardized colon-derived two-dimensional epithelial monolayer. The complex primary human stem cell-derived intestinal epithelium model, termed RepliGut® Planar, was expanded and passaged in a two-dimensional culture which underwent stimulation for 48 h with fecal supernatants (FS) from CC patients (n = 6), UC patients with active disease (n = 6), and healthy subjects (HS) (n = 6). mRNA sequencing of monolayers was performed and cytokine secretion in the basolateral cell culture compartment was measured. The addition of fecal supernatants did not impair the integrity of the colon-derived epithelial monolayer. However, monolayers stimulated with fecal supernatants from CC patients and UC patients presented distinct gene expression patterns. Comparing UC vs. CC, 29 genes were downregulated and 33 genes were upregulated, for CC vs. HS, 17 genes were downregulated and five genes were upregulated, and for UC vs. HS, three genes were downregulated and one gene was upregulated. The addition of FS increased secretion of IL8 with no difference between the study groups. Fecal luminal factors from CC patients and UC patients induce distinct colonic epithelial gene expression patterns, potentially reflecting the disease pathophysiology. The culture of colonic epithelial monolayers with fecal supernatants derived from patients may facilitate the exploration of IBD- and CC-related intestinal microenvironmental and barrier interactions.},

note = {Publisher: Multidisciplinary Digital Publishing Institute},

keywords = {colon cancer, epithelial barrier, epithelial monolayers, inflammatory bowel disease, intestinal microenvironment},

pubstate = {published},

tppubtype = {article}

}