RepliGut® Systems Detailed Technical Information

Congratulations on bringing RepliGut® Systems into your lab. Below you will find all the safety data sheets relevant to the kits. Please bookmark this page for future access to avoid having to rescan the QR code on your product shipping documents or CofA.

Safety Data Sheets

Download Safety Data Sheets for individual products and kit components here:

Part Code
Part Type
Product Name
Document
MED-RGM-100, MED-REG-200
RepliGut Kit Component – Media
RepliGut Growth Medium – 100mL, 200mL
RepliGut Growth Medium SDS_27JUN2023
MED-RMM-100, MED-RGM-200
RepliGut Kit Component – Media
RepliGut Maturation Medium – 100mL, 200mL
RepliGut Maturation Medium SDS_27JUN2023
HISC-DUO/DX, HISC-JEJ/DX, HISC-ILE/DX, HISC-AC/DX, HISC-TC/DX, HISC-DC/DX
RepliGut Kit Component – Cells
Human Intestinal Epithelial Cells – All regions
RepliGut Planar Human Intestinal Epithelial Cells
IN-CDS
RepliGut Kit Component – Kit Materials
Kit Materials – Cell Dissocation Solution
RepliGut Planar Cell Dissociation Solution
HMUC-TC/DX
Individual Microfuge Tubes
Mucus from Human Intestinal Epithelial Cells
Mucus from Human Intestinal Epithelial Cells

Protocols

Download protocols for successful plating and culturing of RepliGut® Planar

Note the HISC cell lot number you received to ensure you download the correct corresponding protocol. HISC cell lots ending with 1### do not require a cell dissociation step.

SKU
Product Type
Product Name
Protocol Download
RGP-12W-DUO
RGP-12W-JEJ
RGP-12W-TC
RGP-12W-AC
RGP-12W-DC
RepliGut® Kit 12-well formats for all regions
RepliGut Planar – 12 Well – Small Intestine – Duodenum
RepliGut Planar – 12 Well – Small Intestine – Jejunum
RepliGut Planar – 12 Well – Transverse Colon
RepliGut Planar – 12 Well – Ascending Colon
RepliGut Planar – 12 Well – Descending Colon
For cell lots with 0###, use:

For cell lots with 1###, use:
RGP-96W-DUO
RGP-96W-JEJ
RGP-96W-TC
RGP-96W-AC
RGP-96W-DC
RGP-96W-JEJ-T
RGP-96W-TC-T
RepliGut® Kit 96-well formats for all regions
RepliGut Planar – 96 Well – Small Intestine – Duodenum
RepliGut Planar – 96 Well – Small Intestine – Jejunum
RepliGut Planar – 96 Well – Transverse Colon
RepliGut Planar – 96 Well – Ascending Colon
RepliGut Planar – 96 Well – Descending Colon
RepliGut Planar – 96 Well – Small Intestine – Jejunum – Trial Kit
RepliGut Planar – 96 Well – Transverse Colon – Trial Kit
For cell lots with 0###, use:

For cell lots with 1###, use:
HMUC-TC/D5
Individual Cryovial
RepliGut Mucus – Transverse Colon 0.5mL

Download application protocols for use with RepliGut® Planar models

Look here for protocols for key applications of RepliGut® Models. Check back often for additional protocols to be released.

Application
Protocol Number
Protocol Download
RepliGut® Planar Immunostaining
AB-C-011_v2
RepliGut® Planar Lucifer Yellow Permeability Assay
AB-C-014_v3
RepliGut® Planar 96-well EVOM TEER Recording
AB-C-018_v1

FAQ

View our frequently asked questions for answers about RepliGut® Kits and RepliGut® Model performance.  Have a question that is not addressed?  Please reach out to our Scientific Support: scientificsupport@altisbiosystems.com

All RepliGut® Kits
Frequently Asked Questions

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