Publications

@article{ahmad_optimization_2014,

title = {Optimization of 3-D organotypic primary colonic cultures for organ-on-chip applications},

author = {Asad A. Ahmad and Yuli Wang and Adam D. Gracz and Christopher E. Sims and Scott T. Magness and Nancy L. Allbritton},

url = {https://doi.org/10.1186/1754-1611-8-9},

doi = {10.1186/1754-1611-8-9},

issn = {1754-1611},

year  = {2014},

date = {2014-04-01},

urldate = {2023-02-20},

volume = {8},

number = {1},

pages = {9},

abstract = {New advances enable long-term organotypic culture of colonic epithelial stem cells that develop into structures known as colonoids. Colonoids represent a primary tissue source acting as a potential starting material for development of an in vitro model of the colon. Key features of colonic crypt isolation and subsequent colonoid culture have not been systematically optimized compromising efficiency and reproducibility. Here murine crypt isolation yield and quality are optimized, and colonoid culture efficiency measured in microfabricated culture devices.},

keywords = {{PDMS}, {PDMS} Substrate, Colonic Crypt, Crypt Cell, Differentiate Cell Lineage},

pubstate = {published},

tppubtype = {article}

}